Multifaceted Studies of the DNA Interactions and In Vitro Cytotoxicity of Anticancer Polyaromatic Platinum(II) Complexes |
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| Authors: | Benjamin J. Pages Dr. Jennette Sakoff Dr. Jayne Gilbert Prof. Alison Rodger Dr. Nikola P. Chmel Nykola C. Jones Dr. Sharon M. Kelly Dale L. Ang Prof. Janice R. Aldrich‐Wright |
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| Affiliation: | 1. Nanoscale Organisation and Dynamics Group, Western Sydney University, Campbelltown, Australia;2. Calvary Mater Newcastle, Waratah, Australia;3. Department of Chemistry, University of Warwick, Coventry, West Midlands, UK;4. ISA, Department of Physics and Astronomy, Aarhus University, Aarhus, Denmark;5. Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK |
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| Abstract: | This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(PL)(SS‐dach)]Cl2, where PL is a polyaromatic ligand and SS‐dach is 1S,2S‐diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ‐G2 cells, with some submicromolar IC50 values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6‐dimethyl‐1,10‐phenanthroline and 2,2′‐bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity. |
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| Keywords: | Anticancer agents biophysical chemistry DNA platinum spectroscopy |
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