| 毛细管等电聚焦和电渗泵驱动聚焦区带分离蛋白质 |
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| 引用本文： | 舒馨,陈令新,马继平,关亚风. 毛细管等电聚焦和电渗泵驱动聚焦区带分离蛋白质[J]. 分析试验室, 2004, 23(5): 1-4 |
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| 作者姓名： | 舒馨 陈令新 马继平 关亚风 |
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| 作者单位： | 中国科学院大连化学物理研究所,大连,116012;中国科学院大连化学物理研究所,大连,116012;中国科学院大连化学物理研究所,大连,116012;中国科学院大连化学物理研究所,大连,116012 |
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| 基金项目： | 国家自然科学基金重大资助项目(批准号20299030)，国家自然科学基金杰出青年基金资助项目(批准号29925514) |
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| 摘 要： | 建立了一种利用电渗泵驱动毛细管内的聚焦区带,实现毛细管电泳等电聚焦分离蛋白质的方法。通过控制电压来调节泵的输出流量,从而调节聚焦区带的迁移速度。适用于毛细管电泳等电聚焦两步法分离蛋白质等两性物质。考察了对牛血清白蛋白和溶菌酶两种粗提蛋白质混合物的分离,迁移时间的RSD分别为1.6%和1.3%,峰面积的RSD均为1.6%,证明方法可行。
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| 关 键 词： | 毛细管电泳等电聚焦 蛋白质 分离分析 电渗泵 |
| 文章编号： | 1000-0720(2004)05-0001-04 |
| 修稿时间： | 2003-07-05 |
Separation of proteins by capillary isoelectric focusing and electroosmotic pump driving |
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| CHEN Ling-xin,MA Ji-ping and GUAN Ya-feng. Separation of proteins by capillary isoelectric focusing and electroosmotic pump driving[J]. Chinese Journal of Analysis Laboratory, 2004, 23(5): 1-4 |
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| Authors: | CHEN Ling-xin MA Ji-ping GUAN Ya-feng |
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| Abstract: | A. two-step capillary isoelectric focusing and detection was developed for proteins separation. An electroosmotic pump (EOP) was used to migrate the focused zones in the capillary. The output flow rate of the EOP was controlled by the applied voltage, which intuna controlled the migration rate accordingly. A test sample containing a protein mixture of coarse bovine serum albumin (BSA) and lysozyme (Lys) was separated by using this method. The RSD of migrate time was1.6% for BSA and 1.3% for Lys, and the RSD of peak area was 1.6 % for both BSA and Lys. |
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| Keywords: | Capillary isoelectric focusing Protein Separation and analysis Electroosmotic pump |
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