Direct observation of single molecule conformational change of tight-turn paperclip DNA triplex in solution |
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| Authors: | Liu Ching-Ping Wey Ming-Tsai Chang Chia-Ching Kan Lou-Sing |
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| Institution: | (1) Institute of Chemistry, Academia Sinica, Taipei, 11529, Taiwan;(2) College of Life Science, National Tsinghua University, Hsinchu, 300, Taiwan;(3) Department of Biological Sciences and Technology, National Chiao Tung University, 75 Po-Ai Street, Hsinchu, 30050, Taiwan;(4) Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan;(5) Department of Bioengineering, Tatung University, Taipei, 104, Taiwan; |
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| Abstract: | DNA triplex modulates gene expression by forming stable conformation in physiological condition. However, it is not feasible
to observe this unique molecular structure of large molecule with 54 oligodeoxynucleotides directly by conventional nuclear
magnetic approach. In this study, we observed directly single molecular images of paperclip DNA triplexes formation in a buffer
solution of pH 6.0 by atomic force microscopy (AFM). Meanwhile, a diffuse “tail” of unwound DNA was observed in pH 8.0 solution.
This designable approach in visualizing the overall structures and shapes of oligo-DNAs at the single molecular level, by
AFM, is applicable to other biopolymers as well. |
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