Synthesis and the reversed-phase HPLC analysis ofcis andtrans-dichlorobis(ethylenediamine-ruthenium) chloride isomers |
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Authors: | N S Gonçalves L C G Vasconcellos S E Mazzetto |
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Institution: | (1) Departmento de Química Organica e Inorganica, Universidade Federal do Ceará, Caixa Postal 12.200, CEP: 60455-760 Fortaleza, CE, Brazil |
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Abstract: | Summary In this work we report the monitoring of the synthesis ofcis andtrans-dichlorobis(ethylenedia-mine)ruthenium chloride isomers by HPLC. The preparation of thecis andtrans complexes were as described in the literature with modifications arising from reversed-phase HPLC results. Thecis andtrans complexes were separated with retention times of 3.0 min and 5.5 min respectively using an ODS column (250 mm×4.6 mm i.d.
5 μm particles, Alltech) and methanol/water 45/55 ratio as mobile phase. According to HPLC results the reaction time to completion
is 48 h and not 72 h as previously described. The shorter time resulted in better yield 82% and reduced by-products. |
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Keywords: | Column liquid chromatography Reaction monitoring cis andtans-Dichlorobis(ethylenediamine-ruthenium) chloride |
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