On-chip micellar electrokinetic chromatographic separation of phenolic chemicals in waters |
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Authors: | Wakida Shin-ichi Fujimoto Kazushi Nagai Hidenori Miyado Takashi Shibutani Yasuhiko Takeda Sahori |
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Institution: | Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. s.wakida@aist.go.jp |
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Abstract: | This paper describes on-chip micellar electrokinetic chromatography (MEKC) separation of bisphenol A and 3 kinds of alkylphenols, which have been recently recognized as endocrine disrupting chemicals for fish by the Japanese government, using microchip capillary electrophoresis with UV detection. We successfully obtained high-speed separation of the phenolic chemicals within 15 s as optimizing in microfluidic controls and MEKC separation conditions. We obtained fairly good linearity with correlation coefficient of over 0.98 from 0 to 50 mg/l phenolic chemicals except for 4-nonylphenol, which sample is the mixture of many geometrical isomers (r = 0.86). The values of the relative standard deviation for peak height in 50 mg/l phenolic chemicals were less than 8% except for bisphenol A (11.0%). The limits of detection obtained at a signal-to-noise ratio of 3 were from 5.6 to 20.0 mg/l. To realize on-site monitoring, we described strategy for on-chip MEKC analysis of the phenolic chemicals in waters using a portable analyzer based on microfluidic devices. |
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