Effect of surface charge on adsorption of bovine serum albumin 2. Interaction of protein molecules with an anionic monolayer,as studied by ellipsometry,radiotracer and surface tension measurements |
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Authors: | N Watanabe T Shirakawa M Iwahashi T Seimiya |
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Institution: | (1) Present address: Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Tokyo, Japan;(2) Toyo Ink Mfg. Co., Ltd., Itabashi, Tokyo, Japan;(3) Department of Chemistry, Faculty of Hygienic Science, Kitasato University, Kanagawa, Japan |
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Abstract: | The adsorption of bovine serum albumin (BSA) onto an anionic monolayer of sodium docosylsulfate (SDocS) spread at the air/water interface was studied by ellipsometry. The adsorption behavior of BSA was estimated from the observed changes in phase differences and in the ratio of reflection coefficients. The dynamic process of BSA adsorption was measured after the injection of BSA solution into the aqueous substrate of SDocS monolayer. The gentle stirring of the substrate solution for 10 min was found to be enough to make the solution homogeneous without damaging the monolayer. The adsorption characteristics of BSA onto a negatively charged surface was compared with that onto a positively charged surface previously reported.The amount of adsorption depended on time and showed a maximum with an initial rapid rise, followed by gradual decrease toward the ultimate equilibrium value. The amount and time of the maximum adsorption depended on the concentration of BSA added to the aqueous substrate.Separate radiotracer measurement, using35S-labeled SDocS monolayer, which is insoluble by itself, revealed that SDocS is solubilized into the bulk solution when BSA is added to the aqueous substrate. |
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Keywords: | Ellipsometry radio tracer bovine serum albumin protein adsorption anionicmonolayer desorption ofmonolayer |
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