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Comparative genomics for understanding the structure,function and sub-cellular localization of hypothetical proteins in Thermanerovibrio acidaminovorans DSM 6589 (tai)
Institution:1. National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440020, Maharashtra, India;2. Department of Microbiology, S. K. Porwal College, Kamptee 441001, Maharashtra, India;1. Institute of Physical Chemistry, Polish Academy of Sciences, Kasprazaka 44/52, Warsaw, 01-224, Poland;2. Department of Chemistry, Kindai University, Higashi, Osaka, 577-8502, Japan;3. Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR - UMR 6226, F-35000, Rennes, France;4. Université Paris-Saclay, CNRS, Institut des Sciences Moléculaires d’Orsay, 91405, Orsay, France;1. Department of Mechanics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China;2. Lanzhou University, Lanzhou 730000, China;1. Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Instituto de Investigação e Formação Avançada (IIFA), Universidade de Évora, Ap. 94, 7002-554 Évora, Portugal;2. Departamento de Biologia, Escola de Ciências e Tecnologia, Universidade de Évora, Polo da Mitra, Ap. 94, 7002-554 Évora, Portugal
Abstract:The Thermanerovibrio acidaminovorans DSM 6589 (tai) is a unique bacterium isolated from anaerobic sludge bed reactor from sugar refinery in Netherland. The comparative genomic studies for understanding the hypothetical proteins in T. acidaminovorans DSM 6589 (tai) were carried out using different bioinformatic tools and web servers. In all 320 hypothetical proteins were screened from the total available genome. The Insilico function prediction for 320 hypothetical proteins was achieved by using different online servers like CDD-Blast, Interproscan and pfam whereas, the structure prediction for 202 hypothetical proteins were deciphered by using protein structure prediction server (PS2 server). The sub-cellular localization for the identified proteins was predicted by the use of cello v2.5 for 320. The study carried out has helped us to understand the structures and functions of unknown proteins available in T. acidaminovorans DSM 6589 (tai) through comparative genomic approach.
Keywords:Anaerobic sludge bed reactor  Sugar refinery  Bioinformatics  Annotation  Hypothetical  Interproscan
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