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Effect of lignocellulosic degradation compounds from steam explosion pretreatment on ethanol fermentation by thermotolerant yeast Kluyveromyces marxianus
Authors:Jose Miguel Oliva  Felicia Sáez  Ignacio Ballesteros  Alberto González  Maria José Negro  Paloma Manzanares  Mercedes Ballesteros
Affiliation:(1) CIEMAT-Departmento de Energías Renovables, Av. Complutense, 22, 28040 Madrid, Spain
Abstract:The filtrate from steam-pretreated poplar was analyzed to identify degradation compounds. The effect of selected compounds on growth and ethanolic fermentation of the thermotolerant yeast strain Kluyveromyces marxianus CECT 10875 was tested. Several fermentations on glucose medium, containing individual inhibitory compounds found in the hydrolysate, were carried out. The degree of inhibition on yeast strain growth and ethanolic fermentation was determined. At concentrations found in the prehy-drolysate, none of the individual compounds significantly affected the fermentation. For all tested compounds, growth was inhibited to a lesser extent than ethanol production. Lower concentrations of catechol (0.96 g/L) and 4-hydroxybenzaldehyde (1.02 g/L) were required to produce the 50% reduction in cell mass in comparison to other tested compounds.
Keywords:Ethanol production   Kluyveromyces marxianus   poplar biomass  inhibitors  fermentation
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