Identification and characterization of supercritical fluid extracts of Rhizoma Chuanxiong by high performance liquid chromatography ion trap mass spectrometry |
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| Authors: | Jiuwei Teng Hongwei Chen Deliang Li Andong Luo |
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| Institution: | (1) Guangdong Key Laboratory of Chemical Emergency Test, China National Analytical Center, Guangzhou, 510070, China;(2) Institute of Analytical Chemistry, School of Chemistry & Environment, South China Normal University, Guangzhou, 510006, China;(3) Guangzhou Honsea Sunsine Bio Science&Technology Co., Guangzhou, 510620, China |
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| Abstract: | The main constituents, senkyunolide A, Z-ligustilide, neocnidilide, 3-butylphthalide, and ligustilide dimers, in supercritical
CO2 fluid extracts of Rhizoma Chuanxiong, a popular Chinese traditional medicine, have been identified and characterized by high
performance liquid chromatography tandem mass spectrometry. Separations were carried out on an Agilent (ECLIPSE XDB) C18 analytical
column by gradient elution with 0.25% acetic acid and methanol (containing 0.25% acetic acid). An Agilent 1100 series LC/MSD
XCT system was operated under positive ESI and auto MS/MS modes for mass spectrometric analysis. Collision-induced dissociation
(CID) fragmentations of these phthalides have been investigated and elucidated. Phthalides have primarily undergone two ESI
CID pathways: side-chain cleavage with losses of alkenes and ring-opening with eliminations of H2O followed by losses of CO. Direct neutral loss of CO has not been observed. Sodium adduct ions have demonstrated completely
different CID pathways.
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Translated from Journal Instrumental Analysis (in press, in Chinese) |
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| Keywords: | HPLC-MS/MS Rhizoma Chuanxiong ligustilide CID supercritical fluid extraction |
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