Molecular encapsulation of Valganciclovir by β-cyclodextrin influences its binding to bovine serum albumin: a spectroscopic study |
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Authors: | Sudha Natesan |
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Institution: | Nanotoxicology Research Lab, School of Engineering and Technology, Karunya University, Coimbatore, India |
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Abstract: | We report, in this paper, the stoichiometry, the binding constant and the structure of the β-cyclodextrin complex of the famous drug Valganciclovir. We investigate the influence of the complex formation of Valganciclovir with β-cyclodextrin, in the binding strength of the drug to the model carrier protein bovine serum albumin. Based on the electronic absorption, fluorescence and 2D rotating-frame Overhauser effect spectroscopy (ROESY) NMR spectral data, it follows that Valganciclovir forms a 1:1 complex with β-cyclodextrin. The β-CD molecule encapsulates the aliphatic chain of the substituted ester molecule. The association constant value of the drug-protein binding in the presence of β-cyclodextrin decreases from that in the absence of β-cyclodextrin, i.e., from that in the case of free drug, i.e. from 3.99 × 104 M?1 to 5.21 × 103 M?1. We compare the results of the binding of the drug to bovine serum albumin in free- and β-cyclodextrin-complex forms. |
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Keywords: | Valganciclovir serum albumin fluorescence 2D ROESY β-cyclodextrin |
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