Synthesis,cytotoxicity, apoptosis and cell cycle arrest of a monoruthenium(II)-substituted Dawson polyoxotungstate |
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| Authors: | Shi-Fang Jia Xiu-Li Hao Yan-Zhen Wen |
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| Institution: | School of Chemical and Biological Engineering, Tai Yuan University of Science and Technology, Taiyuan, China |
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| Abstract: | By 5-h reaction of cis-RuIICl2(DMSO)4] (M2) with K10α2-P2W17O61] (M3) in ice-cooled, HCl-acidic aqueous solution, a water-soluble 1:2-type diamagnetic ruthenium(II) complex of formula K18RuII(DMSO)2(P2W17O61)2]·35H2O (M1) was unexpectedly obtained as an analytically pure, homogeneous tan-colored solid, in which two DMSO ligands are coordinated to the ruthenium(II) atom. The cytotoxic potential of the complex was tested on C33A, DLD-1, and HepG-2 cancer cells and human normal embryonic lung fibroblasts cell MRC-5; the viability of the treated cells was evaluated by MTT assay. The mode of cell death was assessed by morphological study of DNA damage and apoptosis assays. Compound M1 induced cell death in a dose-dependent manner, and the mode of cell death was essentially apoptosis though necrosis was also noticed. Cell cycle analysis by flow cytometry indicated that M1 caused cell cycle arrest and accumulated cells in S phase. | |
| Keywords: | Polyoxometalates Ru(II) complex cytotoxicity cell apoptosis cell cycle arrest |
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