A technique for the synthesis of highly-pure, mono-epitopic, multi-valent lipid core peptide vaccines |
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Authors: | Peter M. Moyle Ning Huang Michael F. Good |
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Affiliation: | a School of Molecular and Microbial Sciences and School of Pharmacy, The University of Queensland, St. Lucia, QLD 4072, Australia b Queensland Institute of Medical Research, Herston, QLD 4029, Australia |
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Abstract: | The synthesis of lipid core peptide (LCP) vaccines using stepwise solid-phase peptide synthesis commonly results in products which are difficult to purify to homogeneity. A new technique for synthesizing highly-pure, mono-epitopic, multi-valent LCP-systems using native chemical ligation is presented. Various conditions were assessed for ligating four copies of a thioester-modified 88/30 serotype group A streptococcal peptide antigen onto an LCP-system featuring four cysteine residues. Overall, the vaccine was synthesized in high purity (>99%), and high yield (90%) when the ligation reaction was performed in the presence of 1% sodium dodecyl sulfate and at elevated temperatures (37 °C). |
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Keywords: | Lipopeptide vaccines Lipid core peptide system Native chemical ligation Peptide vaccines |
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