紫草中活性成分的非极性溶剂动态微波提取及高效液相色谱法测定

用非极性溶剂动态微波辅助提取,高效液相色谱法测定紫草中的紫草素和β,β′-二甲基丙烯酰紫草素.考察了微波吸收介质类型、提取溶剂种类、提取溶剂流速、微波功率和样品粒度对提取产率的影响,优化提取参数.在优化条件下,将所建立的方法与超声提取和索氏提取相比,所得紫草素和β,β′-二甲基丙烯酰紫草素的产率相差不大,但本文所建立的方法所需提取时间短(5min)、溶剂消耗少(10mL).与极性溶剂动态微波辅助提取相比,提取产率大幅度提高.结果说明,所建立的方法是一种有效的提取中草药中一些活性成分的方法,特别是对于一些在非极性溶剂中有更高溶解度的化合物,此方法更具优势.

Determination of the active components in Arnebia eucroma(Royle) Johnst by non-polar solvent dynamic microwave-assisted extraction coupled with HPLC

Non-polar solvent dynamic microwave-assisted extraction（DMAE） was applied to the extraction of shikonin and β,β′-dimethylacrylshikonin in Arnebia eucroma（Royle） Johnst.The exprerimental parameters of non-polar solvent DMAE,including types of microwave absorption media,types of extraction solvents,the flow rate of extraction solvents,microwave irradiation power and size of sample particles were examined and optimized separately.The extraction yields of target compounds obtained by non-polar solvent DMAE（NPSDMAE） were comparable with those obtained by ultrasonic extraction（UE） and soxhlet extraction（SE）.However,when NPSDMAE was applied,short extraction time（5 min） and a small amount of solvent（10 mL） were required.Compared with polar solvent DMAE,the proposed method was demonstrated to obtain higher extraction yield.The experimental results indicate that the proposed method is especially suitable for the compounds,the solubility of which in non-polar solvents is higher than that in polar solvents.