Microdevice for separation and quantitative fraction collection |
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Authors: | Spesný Michal Foret Frantisek |
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Institution: | Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic. |
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Abstract: | A new microfluidic concept for quantitative whole-column fraction collection of electrophoretically separated zones was developed. The prototype device, fabricated on a polycarbonate disk by injection molding, integrated electrophoretic separation channels with fraction collection reservoirs distributed along the separation channel. The microdevice was designed in a CD-like format to use the centrifugal force for moving the liquid in the microchannels. A serpentine shape of the separation channel was selected to create segments for quantitative whole-column fraction collection. The operation was tested with visual monitoring of isotachophoretic separation and collection of cationic dyes. |
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