Cellulase Production by <Emphasis Type="Italic">Streptomyces viridobrunneus</Emphasis> SCPE-09 Using Lignocellulosic Biomass as Inducer Substrate |
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Authors: | Fábio Nuno Marques Da Vinha Mônica Pires Gravina-Oliveira Marcella Novaes Franco Andrew Macrae Elba Pinto da Silva Bon Rodrigo Pires Nascimento Rosalie Reed Rodrigues Coelho |
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Institution: | 1.Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCS,Universidade Federal do Rio de Janeiro (UFRJ),Rio de Janeiro,Brazil;2.Centro de Ciências Agrárias, Ambientais e Biológicas,Universidade Federal do Rec?ncavo da Bahia (UFRB),Cruz das Almas,BA,Brazil;3.Instituto de Química, CT,Universidade Federal do Rio de Janeiro (UFRJ),Rio de Janeiro,Brazil |
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Abstract: | An actinomycete strain, isolated from a soil sample under a sugar cane plantation in Brazil and identified as Streptomyces viridobrunneus SCPE-09, was selected as a promising cellulolytic strain, and tested for its ability to produce cellulases from agro-industrial
residues. Sugar cane bagasse or wheat bran was tested as carbon source, and corn steep liquor tested as nitrogen source. Different
concentrations of carbon and nitrogen were tested using factorial design to identify optimal cellulose production. The results
showed that media containing wheat bran 2.0% (w/v) and corn steep liquid 0.19% (w/v) lead to the highest production, 2.0 U mL−1 of CMCase, obtained on the fifth day of fermentation. The pH and temperature profile showed optimal activity at pH 4.9 and
50°C. As for thermostability, endoglucanases were most tolerant at 50°C, retaining more than 80% of maximal activity even
after 2 h of incubation. Zymogram analyses using supernatant from growth under optimized conditions revealed the presence
of two CMCase bands with apparent molecular masses of 37 and 119 kDa. The combination of pH tolerance and CMCase production
from agro-industrial residues by S. viridobrunneus SCPE-09 offers promise for future bioethanol biotechnologies. |
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