Butane and propane oxidation by engineered cytochrome P450cam |
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| Authors: | Bell Stephen G Stevenson Julie-Anne Boyd Helen D Campbell Sophie Riddle Austin D Orton Erica L Wong Luet-Lok |
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| Institution: | Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK OX1 3QR. |
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| Abstract: | The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidizes butane with a turnover rate of 750 min-1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min-1 with 4% yield. |
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