Rapid identification of new bacterial alcohol dehydrogenases for (R)- and (S)-enantioselective reduction of ss-ketoesters |
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Authors: | Zhang Jie Duetz Wouter A Witholt Bernard Li Zhi |
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Affiliation: | Institute of Biotechnology, ETH-Zurich, Honggerberg, CH-8093 Zurich, Switzerland. |
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Abstract: | New bacterial alcohol dehydrogenases with high and complementary enantioselectivity for the reduction of ethyl 3-keto-4,4,4-trifluorobutyrate 1 and methyl 3-keto-3-(3'-pyridyl)-propionate 3 have been rapidly identified by use of a new methodology consisting of preselection of microorganisms based on degradation ability and high-throughput screening with a miniaturized system coupled with fast analysis of enantioselectivities. |
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