Rapid isocratic HPLC analysis of beta-carotene in red peppers (Capsicum annuum L.) and food preparations |
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| Authors: | M. Weissenberg A. Levy I. Schaeffler E. Menagem M. Barzilai |
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| Affiliation: | (1) Laboratory of Natural Products Chemistry, Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan, Israel |
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| Abstract: | Summary A simple, rapid high-performance liquid chromatography method has been devised in order to separate and quantify β-carotene present in red pepper (Capsicum annuum L.) fruits and food preparations. A reversed-phase isocratic non-aqueous system enables the separation of β-carotene within a few minutes, with detection at 450 nm. The selection of extraction solvents, mild saponification conditions, and chromatographic features, is evaluated and discussed. The method is proposed for rapid screening of large plant populations, plant selection, as well as for food products, and also for nutrition and quality control studies. |
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| Keywords: | Column liquid chromatography β -Carotene Capsanthin and Capsorubin Red peppers |
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