通用型激光诱导荧光微流控芯片分析仪的研制与性能考察

设计和研制了一种通用型激光诱导荧光微流控芯片分析仪.检测部分按共聚焦检测原理设计,采用CCD(电荷耦合器件)监测通道,三维自动调节聚焦,发射波长滤光片可方便地更换以适应多种染料选择,能分别显示进样和分离通道2条电流-时间曲线.考察了该分析仪的检测灵敏度、检测极限和线性范围,显示了分析灵敏度高,检测限低和线性范围宽等特点,在自制注塑型PMMA塑料芯片上实现了φX174Haedi-gesT_dNA片段的分离测定和烟叶act基因PCR产物的分析

Development and Performance of Laser-induced Fluorescence Microfluidic Chip Analyzer

A laser induced fluorescence microfluidic chip system was developed. The detection system was designed according to the confocal principle. Main features of the system include the alternated emission filter in order to select many dyes, and computer controlled focusing Via 3 D micro manipulator and charge coupled device(CCD), and two curves of the current vs. time and two curves of the voltage vs. time for injection channel and separation channel displayed on the computer′s screen. The detection limit reached 6.67×10-13 mol/L for rhodamine 6G. The linear range of detection was 4.02×10-6 mol/L～4.02×10-9mol/L. The high performance of the system was demonstrated by separation and detection of DNA fragments and PCR products on a home made cast molded PMMA microchip.