| 光固定化藻蓝蛋白对体外肝癌细胞7402的抑制作用 |
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| 引用本文： | 关燕清,徐和德,郭宝江.光固定化藻蓝蛋白对体外肝癌细胞7402的抑制作用[J].离子交换与吸附,2000,16(6):547-552. |
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| 作者姓名： | 关燕清 徐和德 郭宝江 |
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| 作者单位： | 1. 华南师范大学生物技术研究所,广州 510631
2. 华南师范大学化学系,广州 510631 |
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| 基金项目： | 广东省自然科学(青年)基金 |
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| 摘 要： | 从钝顶螺旋藻中提取、纯化生物活性蛋白－藻蓝蛋白，采用光固定法固定藻蓝蛋白到组织培养聚苯乙烯（ＰＳｔ）基板上，制备生物材料，研究这种新材料对体外肝癌细胞７４０２的抑制作用。实验表明，初始固定化藻蓝蛋白的浓度为２０μｇ／ｅｗｌｌ时，对７４０２细胞的抑制率达到５５％，浓度继续升高，对癌细胞的抑制率反而下降，当藻蓝蛋白浓度高达０．５ｍｇ／ｗｅｌｌ及１ｍｇ／ｗｅｌｌ时抑制率又回升到５５％、６６％，通过显微镜观察到，在固定高浓度蛋白的情况下，组织增减聚苯乙烯表面上的藻蓝蛋白层对肝癌细胞的贴壁生长有一定的阻害作用，可能提高了对癌细胞的抑制率。
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| 关 键 词： | 藻蓝蛋白 光固定化 肝癌细胞 抑制 肝癌 治疗 |
| 文章编号： | 1001-5493(2000)06-0547-06 |
| 修稿时间： | 2000年4月17日 |
INHIBITION ACTIVITY OF PHYCOCYANIN IMMOBILIZATION BIOMATERIAL ON PROLIFERATION OF LIVER CANCER CELLS 7402 |
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| Guan Yanqing,Guo Baojiang.INHIBITION ACTIVITY OF PHYCOCYANIN IMMOBILIZATION BIOMATERIAL ON PROLIFERATION OF LIVER CANCER CELLS 7402[J].Ion Exchange and Adsorption,2000,16(6):547-552. |
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| Authors: | Guan Yanqing Guo Baojiang |
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| Abstract: | A kind of bioactive protein, phycocyanin, is isolated and purified from Spirulina Platensis. By photo-immobilization, phycocyanin is immobilized on cell culture polystyrene. Inhibition activity of this new biomaterial on proliferation of liver cancer cells is investigated. It was showed that the inhibition activity of photo-immobilized phycocyanin (20(g/well) attained to 55%. Increasing the immobilized-amount of phycocyanin, its inhibition activity to liver cancer cells went down. But when the immobilized-amount of phycocyanin was 0.5mg/well and 1mg/well, inhibition activity turned back to 55% and even 66%. It was clearly observed that the high concentration phycocyanin on the surface of polystyrene matrix interfered with cell adhesion. This may be one of the reasons for its high inhibition activity. |
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| Keywords: | phycocyanin Photo-immobilization Liver cancer cells Inhibition |
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