Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+ |
| |
| Authors: | Dennany Lynn Forster Robert J White Blanaid Smyth Malcolm Rusling James F |
| |
| Institution: | National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland. |
| |
| Abstract: | Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer Os(bpy)2(PVP)10]2+ PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, Ru(bpy)2(PVP)10]2+, and Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
