| 用于测量农药残留的小麦酯酶的选择 |
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| 引用本文： | 肖建军, 华泽钊, 徐斐, 李保国. 用于测量农药残留的小麦酯酶的选择[J]. 分析测试学报, 2002, 21(2): 11-14 |
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| 作者姓名： | 肖建军 华泽钊 徐斐 李保国 |
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| 作者单位： | 上海理工大学食品科学与工程研究所,上海,200093 |
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| 基金项目： | 国家自然科学基金重点项目 (59836240) |
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| 摘 要： | 为研制探测农药残留的生物传感器，研究了农药乐果[O,O-二甲基S－（N－甲基胺基甲酰甲戎）二硫代磷酸酯]对各种小麦植物酯酶的抑制，从小麦中提取植物酶，以农药乐果为抑制剂，采用分光光度法研究了乐果对各种小麦酯酶活性的影响；研究显示，不同品种小麦酯酶对农药乐果的敏感度不同，在所研究的品种中，豫麦39和小麦周9对乐果较敏感，研究结果说明了选择小麦酶源的必要性。
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| 关 键 词： | 农药残留 生物传感器 植物酶 小麦酯酶 有机磷农药 乐果 检测 农产品 酶活性 |
| 文章编号： | 1004-4957(2002)02-0011-04 |
| 修稿时间： | 2001-07-19 |
Selection of Plant - esterase for Detection of Pesticide Residue |
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| XIAO Jian _jun,HUA Ze _ zhao,XU Fei,LI Bao _ guo. Selection of Plant - esterase for Detection of Pesticide Residue[J]. Journal of Instrumental Analysis, 2002, 21(2): 11-14 |
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| Authors: | XIAO Jian _jun HUA Ze _ zhao XU Fei LI Bao _ guo |
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| Abstract: | The inhibition effect of pesticide of dimethoate on various plant _ esterases has been studied to develop a biosensor for detection of pesticide residue. Effect of the inhibitor of dimethoate on activities of plant _ esterases from different kinds of wheats was evaluated by spectrophotometric measurement. The results indicated the inhibiting sensitivity of dimethoate to plant _ esterases from different wheats were not the same. Among the kinds of wheate studied, the esterases from the wheats of Yumai 39 and Zhou 9 are more sensitive than others. It was found that the selection of wheat plant _ esterases for cheap detection of pesticide residue is necessary. |
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| Keywords: | Pesticide residue Biosensor Plant _ esterase |
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