WAVELENGTH DEPENDENCE FOR THE INDUCTION OF BACTERIOPHAGE LAMBDA BY ANTITUMOR AGENT GILVOCARCIN V |
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| Authors: | Rosalie K. Elespuru Victoria M. Hitchins |
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| Affiliation: | Laboratory of Chemical and Physical Carcinogenesis, LBI-Basic Research Program. NCI-Frederick Cancer Research Facility, Frederick, MD 21701, USA;Center for Devices and Radiological Health, FDA Rockville, MD 20857, USA |
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| Abstract: | The wavelength dependence for the induction of a lambda- lacZ fusion phage of E. coli by photo-activated gilvocarcin V was determined using a spot test or a quantitative assay for the lacZ gene product. Suspensions of bacteria and chemical were exposed to radiation of different wavelengths in the region 313-546 nm, using a double grating monochromator. The prophage induction profiles generated were similar to the absorption spectrum of gilvocarcin V in this region, with a peak near 400 nm. The radiation fluence and chemical concentration required for threshold levels of prophage induction exhibited a nearly reciprocal relationship. These results have implications for the therapeutic use of gilvocarcins as antitumor agents. |
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