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Ethanol determination in frozen fruit pulps: an application of quantitative nuclear magnetic resonance
Authors:Wilian da Silva Nunes  Caroline Silva de Oliveira  Glaucia Braz Alcantara
Affiliation:Instituto de Química, Laboratório LP2, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil
Abstract:This study reports the chemical composition of five types of industrial frozen fruit pulps (acerola, cashew, grape, passion fruit and pineapple fruit pulps) and compares them with homemade pulps at two different stages of ripening. The fruit pulps were characterized by analyzing their metabolic profiles and determining their ethanol content using quantitative Nuclear Magnetic Resonance (qNMR). In addition, principal component analysis (PCA) was applied to extract more information from the NMR data. We detected ethanol in all industrial and homemade pulps; and acetic acid in cashew, grape and passion fruit industrial and homemade pulps. The ethanol content in some industrial pulps is above the level recommended by regulatory agencies and is near the levels of some post‐ripened homemade pulps. This study demonstrates that qNMR can be used to rapidly detect ethanol content in frozen fruit pulps and food derivatives. Copyright © 2015 John Wiley & Sons, Ltd.
Keywords:NMR  qNMR  1H NMR  ethanol  fruit pulp  principal component analysis
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