| 高效液相色谱-质谱联用法测定饮料中的苯甲酸含量 |
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| 引用本文： | 洪爱华,尹平河,马义,梁志红. 高效液相色谱-质谱联用法测定饮料中的苯甲酸含量[J]. 光谱实验室, 2011, 28(2): 970-972 |
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| 作者姓名： | 洪爱华 尹平河 马义 梁志红 |
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| 作者单位： | 1. 暨南大学分析测试中心,广州市黄埔大道西601号,510632
2. 暨南大学生物工程研究所,广州市黄埔大道西601号,510632 |
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| 摘 要： | 建立了高效液相色谱-质谱联用法检测饮料中的苯甲酸.选用ZORBAX XDB-C18柱(50mm×2.1mm,3.5μm)为分析柱,流动相为乙腈-水,梯度洗脱,流速为200μL·min-1;质谱条件选用气动辅助电喷雾离子源(ESI),检测方式为负离子多离子反应检测(MRM);苯甲酸校准曲线线性范围为1-500μg/L;方...
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| 关 键 词： | 高效液相色谱-质谱联用法 苯甲酸 饮料 |
Determination of Benzoic Acid in Drink by HPLCMS/MS |
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| HONG Ai-Hua,YIN Ping-He,MA Yi,LIANG Zhi-Hong. Determination of Benzoic Acid in Drink by HPLCMS/MS[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(2): 970-972 |
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| Authors: | HONG Ai-Hua YIN Ping-He MA Yi LIANG Zhi-Hong |
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| Affiliation: | HONG Ai-Hua YIN Ping-He MA Yia LIANG Zhi-Hong(Experimental Technology Center,Jinan University,Guangzhou 510632,P.R.China)a(Bio-Engineering Institute of Jinan University,P.R.China) |
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| Abstract: | A method for the determination of the content of benzoic acid in drink was established by high performance liquid chromatography-mass spectrometry（HPLC-MS）.ZORBAX XDB-C18 column（50mm×2.1mm,3.5μm）was used with a mobile phase of acetonitrile-water at flow rate of 200μL·min-1 and gradient elution.Gas auxiliary electrospray ionization source（ESI） was used to detect the benzoic acid by the negative electrospray ionization and multiple reaction monitoring mode（MRM）.The calibration curve of benzoic acid was linear in the range of 1μg·L-1 to 500μg·L-1,and the detection of limitation was 0.1μg·L-1.The recovery was 92.5%—97.9%,and the RSD was 3.13%—6.94%.The method is accurate,sensitive,and specific,and suitable for determination of benzoic acid in drink. |
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| Keywords: | HPLC-MS/MS Benzoic Acid Drink |
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