| RP-HPLC测定巴豆种仁中佛波醇的含量 |
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| 引用本文： | 郭虎,彭飞,马廉举,姜展,刘新.RP-HPLC测定巴豆种仁中佛波醇的含量[J].光谱实验室,2011,28(2):801-805. |
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| 作者姓名： | 郭虎 彭飞 马廉举 姜展 刘新 |
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| 作者单位： | 1. 重庆医科大学药学院,重庆市渝中区医学院路1号重庆医科大学255号信箱,400016
2. 重庆医科大学药学院,重庆市渝中区医学院路1号重庆医科大学255号信箱,400016;重庆高校药物工程研究中心,重庆市,400016 |
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| 摘 要： | 建立巴豆(Crotan tiglium Linn)种仁中佛波醇的HPLC含量测定方法.采用岛津LC-2010型液相色谱仪,Diamonsil C18分析柱(250mm×4.6mm,5μm),流动相:乙腈-水(梯度洗脱),检测波长:234nm,流速:1mL/min,柱温:25C.在选定色谱条件下佛波醇浓度在6.096-1...
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| 关 键 词： | 佛波醇 巴豆 高效液相色谱法 |
Determination of Phorbol in the Seeds of Croton Tiglium Linn by RP-HPLC |
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| GUO Hu,PENG Fei,MA Lian-Ju,JIANG Zhan,LIU Xin.Determination of Phorbol in the Seeds of Croton Tiglium Linn by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(2):801-805. |
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| Authors: | GUO Hu PENG Fei MA Lian-Ju JIANG Zhan LIU Xin |
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| Institution: | GUO Hua PENG Feia MA Lian-Jua JIANG Zhana LIU Xina,ba(College of Pharmacy,Chongqing Medical University,Chongqing 400016,P.R.China)b(Chongqing Pharmaceutical Engineering Research Center,P.R.China) |
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| Abstract: | The determination of phorbol in the seeds of Croton tiglium linn by HPLC was investigated.Shimadzu LC-2010 HPLC,Diamonsil C18 column（250mm×4.6mm,5μm） were uesd,and the mobile phase was consisted of acetonitrile-water（gradient elution） with the flow rate of 1mL/min.The detection wavelength was 234nm,and the column temperature was 25℃.There was a good linear relationship of phorbol in the range of 6.096—101.600μg/mL（r=0.9999）,while the average standard recovery was 96.40% with RSD of 1.03%.The method is established for determination of phorbol in the seeds of Croton tiglium linn by RP-HPLC,and provides a means for future study. |
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| Keywords: | Phorbol Croton Tiglium Linn HPLC |
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