| HPLC测定人体组织中的奥美定中丙烯酰胺的含量 |
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| 引用本文： | 隋丽丽,孟祥军.HPLC测定人体组织中的奥美定中丙烯酰胺的含量[J].光谱实验室,2011,28(2):654-657. |
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| 作者姓名： | 隋丽丽 孟祥军 |
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| 作者单位： | 沈阳医学院基础医学院,沈阳市黄河北大街146号,110034 |
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| 摘 要： | 高效液相色谱法测定人体组织中的奥美定中丙烯酰胺的含量。采用色谱柱Diamonsil C18（5μm,4.6mm×250mm）;甲醇-水（90∶10）为流动相;流速为0.6mL/min;检测波长210nm;柱温为室温。丙烯酰胺在20—30μg/mL浓度范围内,峰面积与浓度呈良好的线性关系,回归方程为y=19204x-87188,r=0.9997,平均回收率为96.7%。该方法样品处理简单,准确度高,精密度好,适合人体组织中丙烯酰胺含量的测定。
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| 关 键 词： | 高效液相色谱法 人体组织 奥美定 丙烯酰胺 |
Determination of Acrylamide in Amazing of Human Organism by HPLC |
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| SUI Li-Li,MENG Xiang-Jun.Determination of Acrylamide in Amazing of Human Organism by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(2):654-657. |
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| Authors: | SUI Li-Li MENG Xiang-Jun |
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| Institution: | SUI Li-Li MENG Xiang-Jun(Department of Basical Medicine,Shenyang Medical College,Shenyang 110034,P.R.China) |
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| Abstract: | The content of acrylamide in amazing of human organism was determined by HPLC with Diamonsil C18 column（5μm,4.6mm×250mm）,the mobile phase of methanolwater（90∶10） at 210nm,room temperature,and flow rate of 0.6mL/min.Linear range of acrylamide was 20—30μg/mL.Regression equation was y=19204x-87188,r=0.9997.The average recovery was 96.7%.This method is simple,specific,and super-precious for the assay of acrylamide in human organism. |
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| Keywords: | HPLC Human Organism Amazing Acrylamide |
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