| 变色酸2C分光光度法测定血清蛋白质研究 |
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| 引用本文： | 胡秋娈,王春风,胡普州,李全民.变色酸2C分光光度法测定血清蛋白质研究[J].理化检验(化学分册),2005,41(6):398-400. |
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| 作者姓名： | 胡秋娈 王春风 胡普州 李全民 |
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| 作者单位： | 1. 洛阳师范学院化学系,洛阳,471022
2. 河南师范大学化学与环境科学学院,新乡,453002 |
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| 摘 要： | 变色酸2C在pH3.82的Britton-Robinson(B-R)缓冲介质中,能与血清蛋白质形成有色复合物,λmax为560nm,比试剂本身红移约51nm,表观摩尔吸光系数为3.05×105L.mol-1.cm-1,测定蛋白质的线性范围为20~80mg.L-1。应用于人血清样品总蛋白的测定,结果与经典的考马斯亮蓝法一致,而且线性范围宽,操作与重现性都优于考马斯亮蓝法。
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| 关 键 词： | 分光光度法 血清蛋白质 变色酸2C |
| 文章编号： | 1001-4020(2005)06-0398-03 |
| 修稿时间： | 2004年3月12日 |
SPECTROPHOTOMETRIC DETERMINATION OF SERUM PROTEINS WITH CHROMOTROPE 2C |
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| HU Qiu-luan,WANG Chun-feng,HU Pu-zhou,Li Quan-min.SPECTROPHOTOMETRIC DETERMINATION OF SERUM PROTEINS WITH CHROMOTROPE 2C[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2005,41(6):398-400. |
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| Authors: | HU Qiu-luan WANG Chun-feng HU Pu-zhou Li Quan-min |
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| Institution: | HU Qiu-luan~1,WANG Chun-feng~2,HU Pu-zhou~1,LI Quan-min~2 |
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| Abstract: | Serum protein bound with Chromotrope 2C to form a color complex in a medium of pH 3.82, having a absorption maxima at 560 nm with 51 nm of red shift as compared to Chromotrope 2C itself. A molar absorptivity of 3.0510~5Lmol~(-1)cm~(-1) (BSA) and linear range of 20~80 mgL~(-1) for protein were obtained. This method has been used for the determination of the total protein in human serum samples. The results obtained by this method agreed well with those obtained by Coomassie brilliant blue G-250 method. Furthermore, the performance and recovery are surperior to the Coomassie brilliant blue G-250 method. |
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| Keywords: | Spetrophotometry Serum protein Chromotrope 2C |
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