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Raman spectroscopy for quantification of water-to-lipid ratio in phospholipid suspensions |
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| Affiliation: | 1. Institute of Automation and Electrometry, Russian Academy of Sciences, Novosibirsk, 630090, Russia;2. Novosibirsk State University, Novosibirsk, 630090, Russia;1. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale D’Alcontres 31, 98166, Messina, Italy;2. Laboratoire de Chimie Physique, UMR8000, Université Paris Sud, 91405, Orsay cedex, France;3. ISIS Facility, STFC Rutherford Appleton Laboratory, Chilton, Oxon, OX11 0QX, UK;4. Department of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, TX 79409, USA;5. Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA;6. Department of Mathematical and Informatics Sciences, Physical Sciences and Earth Sciences, University of Messina, Viale D’Alcontres 31, 98166, Messina, Italy |
| Abstract: | In this paper, a way of quantification of the water-to-lipid ratio in aqueous suspensions of phospholipid vesicles by Raman spectroscopy is suggested. We measured the ratio of Raman intensities of the OH stretching band and the symmetric CH2-stretching mode for series of water suspensions of different phosphatidylcholines with varied water-to-phospholipid ratio. It is shown that there is a simple linear relation between the ratio of Raman intensities and the water-to-lipid ratio if the number of CH2 groups in the hydrocarbon chains is taken into account. The proportionality coefficient of this relation is determined for the gel and fluid phases of phospholipids. |
| Keywords: | Raman spectroscopy Phospholipids Vesicles Water concentration |
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