Selective determination of caffeine and trigonelline in aqueous extract of green coffee beans by FT-MIR-ATR spectroscopy |
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| Affiliation: | 1. University of Natural Resources and Life Sciences, Vienna, Department of Material Sciences and Process Engineering, Peter-Jordan-Straße 82, 1190 Vienna, Austria;2. University of Natural Resources and Life Sciences, Vienna, Department of Civil Engineering and Natural Hazards, Peter-Jordan-Straße 82, 1190 Vienna, Austria;3. Museum of Natural History, Vienna, Department of Prehistory, Burgring 7, 1010 Vienna, Austria;1. Department of Applied Bioresource Science, The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan;2. Department of Agro-industrial Technology, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Indonesia;3. Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan;4. Forensic Science Laboratory, Ehime Prefectural Police Headquarters, Ehime 790-8573, Japan;5. Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan;1. Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Kasr el Aini St., P.B. 11562, Egypt;2. Leibniz Institute of Plant Biochemistry, Dept. Bioorganic Chemistry, Weinberg 3, 06120 Halle (Saale), Germany;1. PPCCA/Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil;2. DQ/Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil;3. DEMEC/Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil |
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| Abstract: | A non-destructive, fast, simple and reliable Fourier transform mid-infrared attenuated total reflectance spectroscopy (FT-MIR-ATR) method for the selective determination of caffeine and trigonelline in the aqueous extract of green coffee beans was developed and validated. The calibration curves were linear in the range 2000 − 7000 mg/L for caffeine and trigonelline with R2 ≥ 0.9997. The limits of detection (LOD) were 140 and 100 mg/L and the limits of quantification (LOQ) were 470 and 330 mg/L for caffeine and trigonelline, respectively. The precision (% RSD) was 3.0% and 4.3% for caffeine and trigonelline, respectively. The developed method was applied to 20 samples of green coffee beans to determine the two alkaloids. The amount of caffeine and trigonelline in the green coffee beans were found in the range 0.84 − 1.15% (w/w) and 0.83 − 1.13% (w/w), respectively. The accuracy of the developed analytical method was evaluated by spiking standard caffeine and trigonelline to green coffee beans and the average recoveries were 93 ± 5% and 98 ± 4%, respectively. Therefore, the developed FT-MIR-ATR methods can be used for direct determination of the two alkaloids in the green coffee beans. |
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| Keywords: | caffeine trigonelline green coffee beans FT-MIR-ATR |
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