|
|||||
|
|
Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker |
|
| Authors: | Jun Yamamoto Nami Maeda Chiaki Komiya Tomohiro Tanaka Masaya Denda Koji Ebisuno Wataru Nomura Hirokazu Tamamura Youichi Sato Aiko Yamauchi Akira Shigenaga Akira Otaka |
| Institution: | 1. Department of Bioorganic Synthetic Chemistry, Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Tokushima 770-8505, Japan;2. Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062, Japan;3. Department of Pharmaceutical Information Science, Institute of Health Biosciences and Graduate School of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Tokushima 770-8505, Japan;4. JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan |
| Abstract: | A fluoride-responsive (FR) amino acid that induces amide bond cleavage upon the addition of a fluoride was developed, and it was applied to an FR traceable linker. By the use of an alkyne-containing peptide as a model of an alkynylated target protein of a bioactive compound, introduction of the FR traceable linker onto the peptide was achieved. Subsequent fluoride-induced cleavage of the linker followed by labeling of the released peptide derivative was also conducted to examine the potential applicability of the FR traceable linker to the enrichment and labeling of alkynylated target molecules. |
| Keywords: | Cleavable linker Fluoride-responsive Traceable linker Stimulus-responsive amino acid |
| 本文献已被 ScienceDirect 等数据库收录！ | |