Biotransformations of steroids to testololactone by a multifunctional strain Penicillium simplicissimum WY134-2 |
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Authors: | Beibei Yang Yu Wang Xi Chen Jinhui Feng Qiaqing Wu Dunming Zhu Yanhe Ma |
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Institution: | 1. National Engineering Laboratory for Industrial Enzymes and Tianjin Engineering Center for Biocatalytic Technology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;2. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract: | The biotransformations of a range of steroidal compounds, including 17α-hydroxy progesterone, progesterone, testosterone, androst-4-ene-3,17-dione (AD), pregnenolone, and dehydroepiandrosterone (DHEA), by Penicillium simplicissimum WY134-2 have been investigated. In all the cases, testolic acid and testololactone were detected, and the acid was converted to the lactone when pH was adjusted to 1, leading to isolation of testololactone in 25%–96% yields. Especially for progesterone and testosterone, the isolated yields were 93% and 96% with substrate concentration being 3 g/L, suggesting that P. simplicissimum WY134-2 may be used for the synthesis of testololactone. The results revealed the multi-functional catalytic activity of P. simplicissimum WY134-2 toward steroids for the first time. The possible reaction pathways of steroids promoted by this strain were discussed. |
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Keywords: | Steroids Biotransformation Testolactone Testolic acid Penicillium simplicissimum Metabolic pathways |
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