Conformation of the glycosidic linkage in a disaccharide investigated by double-quantum solid-state NMR. |
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Authors: | S Ravindranathan T Karlsson K Lycknert G Widmalm M H Levitt |
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Affiliation: | Physical Chemistry Division, Stockholm University, Stockholm, S-10691, Sweden. |
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Abstract: | Double-quantum heteronuclear local field NMR is performed on a sample of a 13C2-labeled disaccharide, in which the two 13C spins are located on opposite sides of the glycosidic linkage. The evolution of the double-quantum coherences is found to be consistent with the solid-state conformation of the molecule, as previously determined by X-ray diffraction. The dependence of the double-quantum evolution on the glycosidic torsional angles is examined by using a graphical molecular manipulation program interfaced to a numerical spin simulation module. |
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