基于铂/碳球的乙酰胆碱酯酶传感器在农药乐果检测中的应用研究

基于农药乐果对乙酰胆碱酯酶的抑制作用,构建生物传感器,实现了农药乐果的快速、高灵敏检测。合成了纳米材料铂/碳球(Pt/Cs),利用其比表面积大、导电性好的优势,构建乙酰胆碱酯酶(ACh E)传感器。铂/碳球修饰电极比裸电极的阻抗更低,峰电流增加了147.06%,说明该材料能很好地保持酶的催化活性。在最优实验条件下,用ACh E传感器检测农药乐果,在1.0×10~(-9)~1.0×10~(-6)g/L范围,乐果浓度的负对数与抑制率呈良好的线性关系,其检出限为7.3×10~(-12)g/L(按抑制率为10%计算)。对纺织品样品进行加标回收实验,测得回收率为86.2%~101.7%。

Acetylcholinesterase Biosensor Based on Pt/Carbon Spheres for the Detection of Dimethoate

A biosensor for dimethoate was prepared by exploiting their inhibitory effect on the activity of acetylcholinesterase(AChE).Carbon/Pt sphere material with good chemical stability, large surface area and good conductivity was used to modify the electrode.Compared to a bare electrode, the electron transfer resistance of the prepared electrode is lower,and its peak current is increased by 147.06%.This is ascribed to the larger surface of the Carbon/Pt ball material which could improve theadsorption for AChE and enhance its activity.Under the optimal experimental conditions , the concentration of negative logarithm of dimethoate and inhibition ratio showed a good linear relationship from 1.0×10-9 g/L to 1.0×10-6 g/L.The detection limit was 7.3×10-12g/L(the inhibition rate was 10%).The spiked recoveries for textile samples were in the range of 86.2%-101.7%.The method may provide a new way to fabricate highly sensitive biosensors for the detection of organophophate pesticides in textile.