Synthetic Membrane Technology Sessions,Instrumentation Symposium |
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Authors: | Dominique Adamski-Medda Quang Trong Nguyen Edith Dellacherie |
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Institution: | Laboratoire de Chimie-Physique Macromoléculaire, ERA-CNRS No. 23, E.N.S.I.C. 1, rue Grandville, 54042, Nancy Cedex France |
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Abstract: | When a protein is exposed to a ligand linked to a water soluble polymer, a macrocomplex can be formed that can be isolated from the contaminant medium by different methods such as affinity partitioning or gel filtration. Ultrafiltration technique has also been tested in the separation of trypsin and chymotrypsin using a high molecular weight dextran—p-aminobenzamidine as a macroligand. Some theoretical considerations are given in order to evaluate the performance that can be expected from this biospecific complexation—ultrafiltration technique in the separation of biological species. |
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