| 黑海参多糖组成的研究 |
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| 引用本文： | 李熙灿,曾和平,杨世柱.黑海参多糖组成的研究[J].广州化学,1998(1). |
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| 作者姓名： | 李熙灿 曾和平 杨世柱 |
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| 作者单位： | 广州中医药大学中药学院(李熙灿)，华南师范大学化学系(曾和平，杨世柱) |
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| 摘 要： | 通过酶降解、酒精沉淀、DEAE柱纯化和脱色精制四步，从黑海参体壁中制得黑海参多糖的纯品。经电泳法及凝胶拉法鉴定，所得样品为均一组分，是一种典型的动物酸性杂多糖，内含硫酸酯基。样品经水解后用HPLC检测，得葡萄糖（36．7％）、岩藻糖（16．4％）、葡萄糖醛酸（34．2％）、木糖（7．22％）及一些未知成分（5．47％）。经Smith降解及一些波谱特征表明，单糖之间苷键是α-型，主要为1-2和1-3连结。凝胶往法测定其平均分子量约是96×104。
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| 关 键 词： | 黑海参多糖 酸水解 Smith降解 |
The Studies on the Composition of Polysaccharide of Holothuria atra |
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| Li,Xican.The Studies on the Composition of Polysaccharide of Holothuria atra[J].Guangzhou Chemistry,1998(1). |
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| Authors: | Li Xican |
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| Abstract: | PHA (Polysaccharide of Holothuria atra ) has been obtained from the body wallof Holothuriaatra(Jeoper), by enzymic degradation , precipitation on alcohol purification on DEAE-cellulosecolumn and decoloration. PHA is a typical animal acidic heterpolysac-charide .The polysaccharide PHA was shown to be pare both by electrophoresis and by gel chromatography . The results of complete acidic hydrolysis and HPIC analysis indicated that PHA iscomposed of glucose (36. 7% ), fucose(16. 4% ), glucuronic acid (34. 2% ), xylose (7 - 22% ), estersulphate and some unknown constituenes (5. 47% ). Smith degration and wave spectrums (IR,13 C-NMR, etc. ) show that sugare are mainly linked by α-, 1-2 or 1-3 glucosides.The averageM.W. is 96000. |
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| Keywords: | holothuria atra polysaccharide acidic hydrolysis smith degradation |
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