| 阿魏酸哌嗪与牛血清白蛋白相互作用的研究 |
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| 引用本文： | 冯素玲,袁道琴. 阿魏酸哌嗪与牛血清白蛋白相互作用的研究[J]. 分析试验室, 2009, 28(7) |
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| 作者姓名： | 冯素玲 袁道琴 |
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| 作者单位： | 河南师范大学化学与环境科学学院,新乡,453007;河南师范大学化学与环境科学学院,新乡,453007 |
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| 基金项目： | 河南省新乡市科技发展项目 |
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| 摘 要： | 在模拟人体生理条件下,用常规荧光光谱法和紫外-可见吸收光谱法研究阿魏酸哌嗪和牛血清白蛋白结合反应的特征,并利用同步荧光法和三维荧光法研究了阿魏酸哌嗪与牛血清白蛋白作用前后白蛋白的构象变化.研究表明:阿魏酸哌嗪与牛血清白蛋白形成复合物,从而猝灭牛血清白蛋白的内源性荧光.该过程为静态猝灭过程.根据Stem-Volmer方程求出不同温度下的结合位点数和结合常数;根据Fsmter非辐射能量转移理论可求出其作用距离r=2.33nm:根据基本热力学参数△H、△S和△G判断阿魏酸哌嗪和牛血清白蛋白主要通过氢键和范德华力发生相互作用.
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| 关 键 词： | 阿魏酸哌嗪 牛血清白蛋白 荧光光谱 紫外吸收光谱 同步荧光光谱 三维荧光光谱 |
Study on interaction between piperazine ferulate and bovine serum albumin |
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| FENG Su-ling,YUAN Dao-qin. Study on interaction between piperazine ferulate and bovine serum albumin[J]. Chinese Journal of Analysis Laboratory, 2009, 28(7) |
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| Authors: | FENG Su-ling YUAN Dao-qin |
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| Affiliation: | College of Chemistry and Environmental Science;Henan Normal University;Xinxiang 453007 |
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| Abstract: | The interaction between piperazine ferulate and bovine serum albumin was studied at pH 7.4 by spectroscopic methods including fluorescence spectra and UV-visible absorption spectra.Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the structure change of bovine serum albumin with the addition of piperazine ferulate.It showed that the complex formation between bovine serum albumin and piperazine ferulate resulted in the quenching of the intrinsic fluorescence of bov... |
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| Keywords: | Piperazine ferulate Bovine serum albumin Fluorescence spectrum Absorption spectrum Synchronous fluorescence spectrum Three-dimensional fluorescence spectrum |
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