Removal of detergent and solvent from solvent-detergent-treated immunoglobulins. |
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Authors: | A Trescec M Simi? K Branovi? B Gebauer B Benko |
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Institution: | Institute of Immunology, University of Zagreb, Croatia. |
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Abstract: | The solvent-detergent (S/D) method was applied for inactivation of lipid-enveloped viruses during the production of immunoglobulins. Amberlite XAD-7 resin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and detergent (Triton X-100) after the performed S/D inactivation procedure. The S/D reagents from the immunoglobulin preparation were adsorbed on Amberlite XAD-7, while immunoglobulins passed through the column and retained their biological activity. Using the method developed here, the final immunoglobulin preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP. |
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