一锅法合成去甲基麻黄素

以廉价底物苯甲醛、 丙酮酸和NH₄Cl为原料, 通过级联乙醛羟酸合成酶(ASAHⅠ)催化缩合反应和胺脱氢酶(BbAmDH)催化还原胺化反应, 构建了去甲基麻黄素的生物合成新途径. 通过优化分步法和一锅法的反应条件, 使苯甲醛转化率分别达到99%和83%. 采用一锅法, 利用底物补料策略, 有效解除了ASAHI的底物和产物抑制作用, 批次反应的苯甲醛浓度从40 mmol/L提高到100 mmol/L, 苯甲醛转化率从83%提高到90%, 苯甲醛的时空转化率提高了2.7倍.

Synthesis of Norephedrine in One Pot

In this study， a new biosynthetic pathway of norephedrine was constructed， through cascading the carboligation reaction catalyzed by acetohydroxyacid synthase Ⅰ（ASAHI） and reductive amination reaction ca-talysed by amine dehydrogenase（BbAmDH）， the cheap substrate benzaldehyde， pyruvate and NH₄Cl used as raw materials. Optimizing the reaction conditions of the two-step and one-pot method， the conversion rate of benzaldehyde was as high as 99% in the two-step and 83% in one-pot. Utilizing the strategy of feeding in one pot， the substrate and production inhibition effect of ASAHI was successfully relieved， and the benzaldehyde loading was increased to 100 mmol/L from 40 mmol/L in the batch reaction； the benzaldehyde conversion rate was increased to 90% from 83%， and the space-time conversion rate of benzaldehyde was increased by 2.7 times.