| 镓转铁蛋白与HL60细胞的结合 |
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| 引用本文： | 李英奇,刘斌,赵春贵,杨斌盛.镓转铁蛋白与HL60细胞的结合[J].化学学报,2006,64(18):1933-1936. |
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| 作者姓名： | 李英奇 刘斌 赵春贵 杨斌盛 |
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| 作者单位： | 山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006;山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006;山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006;山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006 |
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| 基金项目： | 国家自然科学基金;山西省自然科学基金;山西大学校科研和教改项目 |
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| 摘 要： | 用125I示踪法测定了不同形式的镓转铁蛋白(GaC-Tf, Ga2-Tf, GaC-Tf-FeN)与HL60细胞的结合作用. 结果表明0 ℃、pH＝7.4时GaC-Tf, Ga2-Tf和GaC-Tf-FeN均与细胞膜结合, 表观结合常数分别为(1.2±0.14)×108, (1.1±0.19)×108和(1.8±0.42)×108 L/mol; 同时测定了125I标记的脱铁转铁蛋白(apoTf)与HL60细胞的表观结合常数为(0.97±0.20)×108 L/mol; HL60细胞的平均受体数为(4.8±0.11)×105. 通过体外研究表明, 不同形式的镓转铁蛋白与受体的结合能力次序为apoTf≈GaC-Tf≈Ga2-Tf＜GaC-Tf-FeN.
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| 关 键 词： | 转铁蛋白 镓(Ⅲ) 125I示踪法 HL60细胞 |
| 收稿时间： | 10 17 2005 12:00AM |
| 修稿时间： | 2005-10-172006-05-24 |
Binding of Gallium-Containing Transferrin to HL60 Cells |
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| LI,Ying-Qi,LIU,Bin,ZHAO,Chun-Gui,YANG,Bin-Sheng.Binding of Gallium-Containing Transferrin to HL60 Cells[J].Acta Chimica Sinica,2006,64(18):1933-1936. |
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| Authors: | LI Ying-Qi LIU Bin ZHAO Chun-Gui YANG Bin-Sheng |
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| Institution: | (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006) |
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| Abstract: | The interaction of 125I-labelled various species of transferrin including GaC-Tf, Ga2-Tf and GaC-Tf-FeN with HL60 cells was investigated. The results showed that the proteins bound to membrane of cells at pH＝7.4 and 0 ℃. The association constants were (1.2±0.14)×108, (1.1±0.19)×108 and (1.8±0.42)×108 L/mol for GaC-Tf, Ga2-Tf and GaC-Tf-FeN, respectively. At the same time, the association constant for 125I-labelled apotransferrin was (0.97±0.20)×108 L/mol. There were about (4.8±0.11)×105 transferrin receptors per cell surface. In vitro studies indicated that com-plexes of Ga and transferrin bind to transferrin receptor with the order of apoTf≈GaC-Tf≈Ga2-Tf＜GaC-Tf-FeN in terms of their association constants. |
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| Keywords: | transferrin gallium(III) 125I-labeling HL60 cells |
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