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Liquid chromatographic determination of the species composition of membrane lipids and their derivatives
Authors:V P Pchelkin  A G Vereshchagin
Abstract:This review gives quantitative results of the separation of individual classes of polar glycerolipids of natural and synthetic origin into their individual species. Universal quantitative criteria calculated from the fatty-acid composition of the species obtained or of their mixtures are proposed for determining the degree of reliability of these results. The fractionation of the initial acyl-containing glycerolipids, their N- and O-derivatives with high hydrophobicity, the products of the enzymatic hydrolysis of the native lipids (diacylglycerols, phosphatidic acids) and also the lipophilic O-derivatives of these products is considered. For all these compounds, the results of their separation by the methods of TLC and HPLC both in the form of the adsorption chromatography of the coordination complexes with silver and also in the form of reversed-phase chromatography are discussed. Institute of Plant Physiology of the USSR Academy of Sciences, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 453–477, July–August, 1989.
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