Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana. |
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| Authors: | S Bala G C Uniyal S K Chattopadhyay V Tripathi K V Sashidhara M Kulshrestha R P Sharma S P Jain A K Kukreja S Kumar |
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| Affiliation: | Analytical Instrumentation, Central Institute of Medicinal and Aromatic Plants, Lucknow, India. |
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| Abstract: | A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2'-deacetoxydecinnamoyltaxinine J and 2'-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV-Vis as well as photodiode array detection. |
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