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Colloidal gold based immunochromatographic strip for the simple and sensitive determination of aflatoxin B1 and B2 in corn and rice |
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| Authors: | Lily Ateko Masinde Wei Sheng Xin Xu Yan Zhang Meng Yuan Ivan R Kennedy Shuo Wang |
| Institution: | 1. Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China 2. Faculty of Agriculture and Environment, University of Sydney, Sydney, NSW, 2006, Australia |
| Abstract: | We have developed a simple and fast immunochromatographic test strip for the simultaneous quantitation of aflatoxin B1 and aflatoxin B2 in corn and rice. The strip contains three pads (sample, conjugate, and absorbing pad) and uses the respective polyclonal antibodies immobilized on gold nanoparticles. Matrix interferences were minimized by application of fugacity theory. Clean-up of samples and pre-treatment of strip pads is not required. The visual detection limit is 0.1 ng mL?1, and the process can be completed within 5 min. Out of 113 natural samples, 16 rice and 27 corn samples (38% in total) were aflatoxin positive and the test results were confirmed by HPLC. The strip shows, however, high cross reactivity to aflatoxins G1, G2, and M1. We consider this strip to possess wide applicability because of its ease of use, sensitivity, stability, and low cost. Grain fungal infection often leads to aflatoxin production. A simple sensitive colloidal gold immunochromatographic strip for visual detection of aflatoxins B1 and B2 in corn and rice with detection limit of 0.1 ng mL-1 within 5 min was developed |
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