A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells |
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Authors: | Elzbieta Jastrzebska Sylwia Flis Aleksandra Rakowska Michal Chudy Zenon Jastrzebski Artur Dybko Zbigniew Brzozka |
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Affiliation: | 1. Department of Microbioanalytics, Institute of Biotechnology, Warsaw University of Technology, 00-664, Warsaw, Poland 2. Department of Pharmacology, National Medicines Institute, 00-725, Warsaw, Poland
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Abstract: | We have investigated the response of normal and cancer cells to exposure a combination of celecoxib (Celbx) and 5-fluorouracil (5-FU) using a lab-on-a-chip microfluidic device. Specifically, we have tested the cytotoxic effect of Celbx on normal mouse embryo cells (Balb/c 3T3) and human lung carcinoma cells (A549). The single drugs or their combinations were adjusted to five different concentrations using a concentration gradient generator (CGG) in a single step. The results suggest that Celbx can enhanced the anticancer activity of 5-FU by stronger inhibition of cancer cell growth. We also show that the A549 cancer cells are more sensitive to Celbx than the Balb/c 3T3 normal cells. The results obtained with the microfluidic system were compared to those obtained with a macroscale in vitro cell culture method. In our opinion, the microfluidic system represents a unique approach for an evaluation of cellular response to multidrug exposure that also is more simple than respective microwell plate assays. Figure ? |
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