Natural abundance (17)O NMR spectroscopy of rat brain in vivo |
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Authors: | de Graaf Robin A Brown Peter B Rothman Douglas L Behar Kevin L |
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Affiliation: | Magnetic Resonance Research Center, Yale University, School of Medicine, New Haven, CT 06520-8043, USA. robin.degraaf@yale.edu |
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Abstract: | Oxygen is an abundant element that is present in almost all biologically relevant molecules. NMR observation of oxygen has been relatively limited since the NMR-active isotope, oxygen-17, is only present at a 0.037% natural abundance. Furthermore, as a spin 5/2 nucleus oxygen-17 has a moderately strong quadrupole moment which leads to fairly broad resonances (T(2)=1-4 ms). However, the similarly short T(1) relaxation constants allow substantial signal averaging, whereas the large chemical shift range (>300 ppm) improves the spectral resolution of (17)O NMR. Here it is shown that high-quality, natural abundance (17)O NMR spectra can be obtained from rat brain in vivo at 11.74 T. The chemical shifts and line widths of more than 20 oxygen-containing metabolites are established and the sensitivity and potential for (17)O-enriched NMR studies are estimated. |
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Keywords: | 17O NMR Natural abundance Rat brain High magnetic field |
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