| 用分光光度法测定蜂胶超临界CO2萃取物中总黄酮含量 |
| |
| 引用本文： | 刘伟,李岂凡,张彬,周武,侯宗福.用分光光度法测定蜂胶超临界CO2萃取物中总黄酮含量[J].南昌大学学报(理科版),2007,31(3):276-278. |
| |
| 作者姓名： | 刘伟 李岂凡 张彬 周武 侯宗福 |
| |
| 作者单位： | 1. 南昌大学,食品科学教育部重点实验室,江西,南昌,330031
2. 南昌大学,环境科学与工程学院,江西,南昌,330031 |
| |
| 摘 要： | 利用碱性条件下黄酮与Al3 生成粉红色络合物在可见光区510 nm处有最大吸收的特性,以芦丁为标样,采用分光光度法测定蜂胶超临界CO2萃取物中总黄酮含量,并与HPLC标准方法测定结果进行比较,发现各平行样的测定结果基本一致,最大偏差为268.38 mg/100 g,最小偏差为52.77 mg/100 g.该法简便快捷,结果相对准确,可作为蜂胶超临界CO2萃取物中总黄酮含量快速评估的一种方法.
|
| 关 键 词： | 蜂胶 超临界CO2 总黄酮 分光光度法 分光光度法测定 蜂胶 超临界 萃取物 总黄酮含量 Spectrophotometry Propolis Extract Total 标准方法 评估 快速 测定结果 最小 差为 大偏 发现 比较 HPLC 标样 |
| 文章编号： | 1006-0464（2007）03-0276-03 |
| 修稿时间： | 2007-03-15 |
Determination of Total Flavanoids in the SCF- CO2 Extract of Propolis by Spectrophotometry |
| |
| LIU Wei,LI Qi-fan,ZHANG Bin,ZHOU Wu,HOU Zong-fu.Determination of Total Flavanoids in the SCF- CO2 Extract of Propolis by Spectrophotometry[J].Journal of Nanchang University(Natural Science),2007,31(3):276-278. |
| |
| Authors: | LIU Wei LI Qi-fan ZHANG Bin ZHOU Wu HOU Zong-fu |
| |
| Institution: | a. Key Laboratory of Food Science of MOE; b. School of Envionmental Science and Engineering, Nanchang University, Nanchang 330031, China |
| |
| Abstract: | In this paper the total flavanoids in SCF-CO2 extract of propolis were determined by spectrophotometry at 510 nm with rutin as sample,and the color complex was obtained by the reaction between flavanoids and Al3+ under the condition of alkalescence.The results were compared with those of the parallel samples that were determined by the standard method with HPLC.The results obtained with those two methods are similar with the largest deviation of 268.38 mg/100 g and the smallest deviation of 52.77 mg/100 g.This method is convenient,fast and relatively accurate,and it could be used as a rapid assessment method in the determination of total flavanoids in the extract of propolis with the SCF-CO2. |
| |
| Keywords: | propolis SCF - CO2 total flavanoids spectrophotometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
