| 柱前衍生化-HPLC测定仲丁胺对映体的纯度 |
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| 引用本文： | 张义文,杨丹,谢永美,刘其松,王欣,宋航. 柱前衍生化-HPLC测定仲丁胺对映体的纯度[J]. 分析试验室, 2009, 28(5) |
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| 作者姓名： | 张义文 杨丹 谢永美 刘其松 王欣 宋航 |
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| 作者单位： | 四川大学制药与生物工程系,成都,610065;四川大学制药与生物工程系,成都,610065;四川大学制药与生物工程系,成都,610065;四川大学制药与生物工程系,成都,610065;四川大学制药与生物工程系,成都,610065;四川大学制药与生物工程系,成都,610065 |
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| 摘 要： | 用3, 5-二硝基苯甲酸对仲丁胺进行柱前衍生, 将其衍生物在CHIRALCEL OD-H手性固定相上拆分, 并通过二极管阵列紫外检测器对其衍生物进行检测, 建立了一种拆分仲丁胺消旋体、测定仲丁胺光学纯度的新方法. 以正己烷和乙醇或异丙醇为流动相, 在CHIRALCEL OD-H手性固定相上对仲丁胺衍生物进行了拆分, 并考察了流动相组成和柱温对该对映体衍生物分离的影响, 获得较好的分析条件, 分离因子大于1.2. 非手性试剂柱前衍生化法测定仲丁胺光学纯度与旋光度方法比较, 结果一致. 方法可用于仲丁胺对映体的质量控制.
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| 关 键 词： | 高效液相色谱 柱前衍生化 手性固定相 仲丁胺 光学纯度 |
Determination of enantiomeric purity for 2-butylamine by HPLC combining with pre-column derivation |
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| ZHANG Yi-wen,YANG Dan,XIE Yong-mei,LIU Qi-song,WANG Xin,SONG Hang. Determination of enantiomeric purity for 2-butylamine by HPLC combining with pre-column derivation[J]. Chinese Journal of Analysis Laboratory, 2009, 28(5) |
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| Authors: | ZHANG Yi-wen YANG Dan XIE Yong-mei LIU Qi-song WANG Xin SONG Hang |
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| Affiliation: | Department of Pharmaceutical and Biological Engineering;Sichuan University;Chengdu 610065 |
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| Abstract: | 2-Butylamine was derivatized with 3,5-dinitrobenzoic acid that was the reagent of pre-colume derivatization of high performance liquid chromatography.The derivative was separated by CHIRALCEL OD-H chiral stationary phase,and detected by a Photo-Diode-Array detector.A method for separating the racemate and determining the enantiomeric purity of 2-butylamine was established.Chromatographic model was employed for enantiomeric separation of 2-butylamine derivatives on the CHIRALCEL OD-H chiral colume in hexane/... |
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| Keywords: | High performance liquid chromatography Pre-colume derivatization Chiral stationary phase 2-Butylamine Optical purity |
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