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Comparison of three different configurations of dual ultramicroelectrodes for the decomposition of S-Nitroso-L-glutathione and the direct detection of nitric oxide |
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| Authors: | Achille Nassi Loan To Thi Kim Aurélie Girard Laurent Griscom Florence Razan Sophie Griveau Laurent Thouin Fethi Bedioui |
| Institution: | 1. Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland 2. Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland 3. Department of Clinical Pathology, Medical University of Bialystok, Bialystok, Poland |
| Abstract: | Podoplanin (PDP) is a small transmembrane protein and widely present in various specialized cells throughout the human body. It is a specific marker for identification of lymphatic vessel and a candidate marker for cancer stem cells in squamous cell carcinoma of the lung. We report on method for the highly selective determination of PDP by using a surface plasmon resonance imaging (SPRI) that exploits the highly selective interaction between PDP and anti-human PDP monoclonal antibody (IgG). The sensor has a dynamic range between 0.25 and 1.0?ng?mL?1, and a detection limit of 15?pg?mL?1. It was applied to the determination of PDP in blood plasma and tissue homogenates from paired normal and lung tumor tissue. A novel method for the highly selective determination of podoplanin by using a Surface Plasmon Resonance Imaging (SPRI) technique has been developed. For sensor development, highly selective interaction between podoplanin and anti-human podoplanin monoclonal antibody (IgG) was applied. |
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