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Direct electrochemistry of cytochrome P450 in a biocompatible film composed of an epoxy polymer and acetylene black |
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| Authors: | Chunyan Dai Yonglan Ding Ming Li Junjie Fei |
| Affiliation: | 1. Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, Hunan, People’s Republic of China |
| Abstract: | We report on a composite matrix composed of epoxy copolymers P (GMA-co-MPC) and acetylene black that can be used to entrap cytochrome P450. The composite provides a biocompatible microenviroment and can substantially accelerate the electron transfer between the cytochrome P450 and the electrode. The electrochemical response is characterized by a pair of well-defined redox peaks for the heme Fe(II/III) redox couples were observed at ?483?mV (vs. SCE). The immobilized cytochrome P450 exhibits excellent electrocatalytical activity to diethylstilbestrol (DES). The amperometric response varied linearly with the concentration of DES in the 0.2 to 2.8?μM concentration range. The biosensor displays a detection limit 5.9?×?10-8?mol?L-1 and thus represents a promising candidate for studying the electrochemistry of cytochrome P450s and its sensing applications. Composite matrix of epoxy copolymers P (GMA-co-MPC)) and acetylene black (AB) was firstly used to entrap cytochrome P450 (CYP3A4). The direct electrochemistry behaviors of CYP3A4 were investigated and CYP3A4 immobilized in P(GMA-co-MPC)/AB film exhibited excellent electrocatalytical activity to diethylstilbestrol (DES). CVs of CYP3A4/P(GMA-co-MPC)/AB/GCE. (a) N2-saturated+2μM DES, (b) air-saturated, (c) air-saturated +2?μM DES |
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