利用斑马鱼模型研究芦笋有效成分对免疫功能的调节作用

采用正常发育48 hpf的转基因斑马鱼为实验动物,以氯霉素处理引起免疫抑制,分别加入25.0~200.0 mg/L的芦笋多糖、6.25~100.00 mg/L的芦笋皂苷,处理24 h,观察各组斑马鱼的发育情况,拍照并统计斑马鱼体内中性粒细胞的数量。结果表明,氯霉素处理斑马鱼后,斑马鱼体内中性粒细胞数量减少,芦笋多糖在浓度为50.0~200.0 mg/L范围内对斑马鱼体内中性粒细胞数量减少具有明显改善作用,且具有明显的剂量依赖效应;芦笋皂苷在实验剂量范围内均显示出显著的改善作用。实验结果显示,芦笋的有效成分芦笋多糖、芦笋皂苷对斑马鱼免疫功能具有明显的调节作用。

The immune-regulatory effects of active ingredients of asparagus using zebrafish model

In this experiment, 48 hpf (hour post fertilization) healthy transgenic zebrafish Tg(lyz: EGFP) was used as animal model. Firstly, immune suppression of zebrafish embryos was induced by chloramphenicol treatment. Then those zebrafish embryos were treated with 25.0~200.0 mg/L asparagus polysaccharide and 6.25~100.00 mg/L asparagus saponins for 24 h, respectively. Finally, the development of zebrafish in each group was observed under the microscope,and the number of neutrophils in zebrafish was recorded and counted to evaluate their immune regulatory effects. The results showed that the numbers of neutrophils in 72hpf zebrafish trunk area decreased in chloramphenicol induced group.Asparagus polysaccharide group(50.0~200.0 mg/L) showed a significant immunological enhancement effect with dose dependent manner. Asparagus saponins could also significantly improve chloramphenicol induced immunosuppressive effects within the experimental dose range. In this study, This study showed that the active ingredients of asparagus, such as polysaccharide and asparagus saponin, had obvious regulation effects on the immune function of zebrafish, which provided the scientific basis for further development of asparagus.